In:
Arthritis & Rheumatology, Wiley, Vol. 66, No. 9 ( 2014-09), p. 2545-2557
Kurzfassung:
To examine whether the B cell tropic γ‐herpesvirus Epstein‐Barr virus (EBV) is aberrantly expressed in its latent and lytic forms within ectopic lymphoid structures (ELS) in the salivary glands of patients with Sjögren's syndrome (SS), and to investigate the relationship between EBV dysregulation, B cell activation, in situ differentiation of autoreactive plasma cells, disease‐specific autoantibody production, and cytotoxicity. Methods Latent and lytic EBV infection in the salivary glands of 28 patients with SS and 38 patients with nonspecific chronic sialadenitis (NSCS), characterized for the presence or absence of ELS, was investigated by reverse transcription–polymerase chain reaction, in situ hybridization, and immunohistochemical/immunofluorescence staining. Glandular versus synovial production of anti−Ro 52, anti–citrullinated protein antibodies (ACPAs), and anti–EBV peptide antibodies was analyzed in situ or in vivo in human SS/SCID and human rheumatoid arthritis/SCID mouse chimeras. Results EBV dysregulation within inflammatory infiltrates was observed exclusively in ELS+ SS salivary gland tissue, as revealed by latent EBV infection and lytic EBV infection in B cells and plasma cells, respectively. Conversely, epithelial latent membrane protein 2A expression was observed in both patients with SS and patients with NSCS. Importantly, perifollicular plasma cells displaying Ro 52 immunoreactivity were frequently infected by EBV. Furthermore, ELS‐containing SS salivary gland tissue that was transplanted into SCID mice supported the production of anti–Ro 52/anti–La 48 and anti‐EBV antibodies but not ACPAs. Analysis of CD4+ and CD8+ T cell localization and granzyme B expression demonstrated that the persistence of EBV in ELS‐containing SS salivary glands was associated with follicular exclusion of CD8+ T cells and impaired CD8‐mediated cytotoxicity. Conclusion Active EBV infection is selectively associated with ELS in the salivary glands of patients with SS and appears to contribute to local growth and differentiation of disease‐specific autoreactive B cells.
Materialart:
Online-Ressource
ISSN:
2326-5191
,
2326-5205
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2014
ZDB Id:
2754614-7
