In:
Biotechnology Journal, Wiley, Vol. 14, No. 9 ( 2019-09)
Abstract:
Xylitol is a highly valuable commodity chemical used extensively in the food and pharmaceutical industries. The production of xylitol from d ‐xylose involves a costly and polluting catalytic hydrogenation process. Biotechnological production from lignocellulosic biomass by micro‐organisms like yeasts is a promising option. In this study, xylitol is produced from lignocellulosic biomass by a recombinant strain of Saccharomyces cerevisiae ( S. cerevisiae ) (YPH499‐ Ss XR‐ Aa BGL) expressing cytosolic xylose reductase ( Scheffersomyces stipitis xylose reductase [ Ss XR]), along with a β‐ d ‐glucosidase ( Aspergillus aculeatus β‐glucosidase 1 [ Aa BGL]) displayed on the cell surface. The simultaneous cofermentation of cellobiose/xylose by this strain leads to an ≈2.5‐fold increase in Yxylitol/xylose (=0.54) compared to the use of a glucose/xylose mixture as a substrate. Further improvement in the xylose uptake by the cell is achieved by a broad evaluation of several homologous and heterologous transporters. Homologous maltose transporter ( Sc MAL11) shows the best performance in xylose transport and is used to generate the strain YPH499‐XR‐ Sc MAL11‐BGL with a significantly improved xylitol production capacity from cellobiose/xylose coutilization. This report constitutes a promising proof of concept to further scale up the biorefinery industrial production of xylitol from lignocellulose by combining cell surface and metabolic engineering in S. cerevisiae .
Type of Medium:
Online Resource
ISSN:
1860-6768
,
1860-7314
DOI:
10.1002/biot.201800704
Language:
English
Publisher:
Wiley
Publication Date:
2019
detail.hit.zdb_id:
2214038-4