In:
Biomedical Chromatography, Wiley, Vol. 32, No. 11 ( 2018-11)
Kurzfassung:
Sijunzi decoction (SJZD) is one of the most well‐known traditional Chinese herbal formulations. This study elucidates the pharmacokinetics of SJZD in rat plasma after the administration of a single oral dose of 3 mL/kg using ultra‐high‐performance liquid chromatography electrospray ionization quadrupole–time of flight mass spectrometry (UHPLC‐ESI‐Q‐TOF/MS) with bergapten as an internal standard. The separation was performed on an Agilent Zorbax Eclipse Plus C 18 column (2.1 × 50 mm, 1.8 μm) by elution with acetonitrile and water (containing 0.1% formic acid) at a flow rate of 0.3 mL/min. Electrospray ionization in positive and negative ion modes was used to quantify six compounds, with monitored ion m/z values of 249.1397 [M + H] + and 529.3857 [M + H] + for atractylenolide III (ATL‐III) and pachymic acid (PA), respectively, and m/z of 1107.6638 [M − H] − , 991.5746 [M – H + HCOO] − , 821.3714 [M − H] − , 469.3315 [M − H] − for ginsenoside Rb 1, Re, glycyrrhizic acid (GL), and glycyrrhetinic acid (GA), respectively. The calibration curves for ginsenoside Rb 1 , Re, ATL‐III, PA, GL and GA were 0.0015–0.75, 0.001–0.5, 0.0004–0.2, 0.003–0.9, 0.0015–0.3 and 0.001–1.5 μg/mL, respectively. The intra‐ and inter‐day precisions (RSD) were 〈 14.3%. The rapid, sensitive and specific UHPLC‐ESI‐Q‐TOF/MS method developed and validated in this study was successfully applied to the simultaneous determination of the six components of SJZD using rat plasma for pharmacokinetic studies after oral administration.
Materialart:
Online-Ressource
ISSN:
0269-3879
,
1099-0801
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2018
ZDB Id:
1479945-5
SSG:
12
