In:
Cancer Medicine, Wiley, Vol. 4, No. 1 ( 2015-01), p. 75-83
Abstract:
Immunohistochemistry staining of p53 is a cheap and simple method to detect aberrant function of p53. However, there are some discrepancies between the result of immunohistochemistry staining and mutation analysis. This study attempted to find a new definition of p53 staining by its staining pattern. Immunohistochemistry staining of p53 and TP 53 gene mutation analysis were performed in 148 gastric cancer patients. Also SNP ‐ CGH array analysis was conducted to four cases. Positive staining of p53 was observed in 88 (59.5%) tumors. Tumors with positive p53 staining showed malignant features compared to negative tumors. Mutation of TP 53 gene was observed in 29 (19.6%) tumors with higher age and differentiated type. In positive p53 tumors, two types could be distinguished; aberrant type and scattered type. With comparison to TP 53 gene mutation analysis, all the scattered type had wild‐type TP 53 gene ( P = 0.0003). SNP ‐ CGH array showed that scattered‐type tumors had no change in the structure of chromosome 17. P53‐scattered‐type staining tumors may reflect a functionally active nonmutated TP 53 gene. In interpretation of p53 immunohistochemistry staining, distinguishing p53‐positive tumors by their staining pattern may be important in gastric cancer.
Type of Medium:
Online Resource
ISSN:
2045-7634
,
2045-7634
DOI:
10.1002/cam4.2015.4.issue-1
Language:
English
Publisher:
Wiley
Publication Date:
2015
detail.hit.zdb_id:
2659751-2