In:
Chemistry & Biodiversity, Wiley, Vol. 13, No. 5 ( 2016-05), p. 596-601
Abstract:
Following an in vitro bioactivity‐guided fractionation procedure, 14 compounds including eight flavonoids and six phenylpropanoids were isolated and identified from the AcOEt fraction of Clinopodium chinense ( Benth .) O. Kuntze . All constituents were tested for α ‐glucosidase and high glucose‐induced injury in human umbilical vein endothelial cells ( HUVEC s) inhibitory activities. All constituents exhibited varying degrees α ‐glucosidase inhibitory activity and protective activity on HUVEC s. Among them, luteolin ( 2 ), eriodictyol ( 5 ), ethyl rosmarinate ( 13 ), and clinopodic acids B ( 14 ) were proved to be potent α ‐glucosidase inhibitors with IC 50 value ranging from 0.6 to 2.0 μ m . Additionally, luteolin ( 2 ), naringenin ( 4 ), eriodictyol ( 5 ), ethyl (2 R )‐3‐(3, 4‐dihydroxyphenyl)‐2‐hydroxypropanate ( 9 ), caffeic acid ( 11 ), ethyl rosmarinate ( 13 ), and clinopodic acids B ( 14 ) significantly ameliorate HUVEC s injury induced by high glucose with an approximate EC 50 value of 3 – 36 μ m . These results suggest that the 14 bioactive constituents were responsible for hypoglycemic and protective vascular endothelium effect of C. chinense ( Benth .) O. Kuntze and their structure–activity relationship was also analyzed briefly. Eriodictyol, luteolin, ethyl rosmarinate, and clinopodic acids B were the potential lead compounds of antidiabetic drugs.
Type of Medium:
Online Resource
ISSN:
1612-1872
,
1612-1880
DOI:
10.1002/cbdv.201500187
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
2139001-0
SSG:
12
