In:
Cell Biology International, Wiley, Vol. 39, No. 8 ( 2015-08), p. 899-909
Abstract:
Cell labeling and tracking are becoming increasingly important areas within the field of stem cell transplantation. The ability to track the migration and distribution of implanted cells is critical to understanding the beneficial effects and mechanisms of stem cell therapy. The present study investigated the effects of amine‐surface‐modified superparamagnetic iron oxide (SPIO) nanoparticles on the biological properties of human adipose tissue‐derived stem cells (hADSCs). Monodisperse hydrophobic magnetite (Fe 3 O 4 ) nanoparticles were prepared using silicon and surface‐modified with amine coating. Cell viability, proliferation, differentiation potential, and surface marker expression were evaluated. The magnetic particles (10–18 nm) displayed high labeling efficiency and stability in hADSCs. SPIO‐labeled cells produced a hypointense signal and were effectively visualized by MRI for up to 21 days. The results of MTT proliferation assays and flow cytometry analysis demonstrated that SPIOs were biocompatible, viz. the labeling process did not cause cell death or apoptosis and had no side effects on cell proliferation. In vivo experiments showed that the magnetic particles did not affect liver and kidney function. The successful and stable labeling of hADSCs combined with efficient magnetic tropism demonstrates that SPIOs are promising candidates for hADSC tracking in hADSC‐based cell therapy applications.
Type of Medium:
Online Resource
ISSN:
1065-6995
,
1095-8355
Language:
English
Publisher:
Wiley
Publication Date:
2015
detail.hit.zdb_id:
1462519-2
SSG:
12