In:
ChemMedChem, Wiley, Vol. 16, No. 24 ( 2021-12-14), p. 3739-3749
Abstract:
Functionalized nanoparticles reveal new frontiers in therapeutics and diagnostics, simultaneously referred to as theranostics. Functionalization of an inorganic nanoparticle (NP) with an organic ligand determines the interaction of the functionalized NPs with various cellular components, leading to the desired therapeutic effect, while diminishing adverse side effects. Apart from the therapeutic effect of the nanoparticles, other physical properties of the organic‐inorganic complex (nanohybrid) including fluorescence, X‐ray or MRI contrast offer diagnosis of the anomalous target cell. In this study we functionalized Mn 3 O 4 NPs with organic citrate (C−Mn 3 O 4 ) and folic acid (FA−Mn 3 O 4 ) ligands and investigated their antimicrobial activities using Staphylococcus hominis as a model bacteria, which can be remediated through their membrane rupture. While high‐resolution transmission microscopy (HR‐TEM), XRD, DLS, absorbance and fluorescence spectroscopy were used for structural characterisation of the functionalised NPs, zeta potential measurements and temperature‐dependent reactive oxygen speices (ROS) generation reveal their drug action. We used high‐end density functional theory (DFT) calculations to rationalise the specificity of the drug action of the NPs. Picosecond‐resolved FRET studies confirm the enhanced affinity of FA−Mn 3 O 4 to the bacteria relative to C−Mn 3 O 4 , leading to enhanced antimicrobial activity. We have shown that the functionalised nanoparticles offer significant X‐ray contrast in in‐vitro studies, indicating the FA−Mn 3 O 4 NPs to be a potential theranostic agent against bacterial infection.
Type of Medium:
Online Resource
ISSN:
1860-7179
,
1860-7187
DOI:
10.1002/cmdc.202100494
Language:
English
Publisher:
Wiley
Publication Date:
2021
detail.hit.zdb_id:
2209649-8
SSG:
15,3