In:
ChemSusChem, Wiley, Vol. 16, No. 8 ( 2023-04-21)
Abstract:
l ‐Theanine ( l ‐Th) was synthesized by simply mixing the reactants ( l ‐glutamine and ethylamine in water) at 25 °C and Bacillus subtilis γ‐glutamyl transferase ( Bs GGT) covalently immobilized on glyoxyl‐agarose according to a methodology previously reported by our research group; neither buffers, nor other additives were needed. Ratio of l ‐glutamine (donor) to ethylamine (acceptor), pH, enzymatic units (IU), and reaction time were optimized (molar ratio of donor/acceptor=1 : 8, pH 11.6, 1 IU mL −1 , 6 h), furnishing l ‐Th in 93 % isolated yield (485 mg, 32.3 g L −1 ) and high purity (99 %), after a simple filtration of the immobilized biocatalyst, distillation of the volatiles (unreacted ethylamine) and direct lyophilization. Immobilized Bs GGT was re‐used (four reaction cycles) with 100 % activity retention. This enzymatic synthesis represents a straightforward, fast, high‐yielding, and easily scalable approach to l ‐Th preparation, besides having a favorable green chemistry metrics.
Type of Medium:
Online Resource
ISSN:
1864-5631
,
1864-564X
DOI:
10.1002/cssc.202202108
Language:
English
Publisher:
Wiley
Publication Date:
2023
detail.hit.zdb_id:
2411405-4