In:
Cytometry Part A, Wiley, Vol. 87, No. 4 ( 2015-04), p. 357-368
Kurzfassung:
The recent introduction of mass cytometry, a technique coupling a cell introduction system generating a stream of single cells with mass spectrometry, has greatly increased the number of parameters that can be measured per single cell. As with all new technology there is a need for dissemination of standardization and quality control procedures. Here, we characterize variations in sensitivity observed across the mass range of a mass cytometer, using different lanthanide tags. We observed a five‐fold difference in lanthanide detection over the mass range and demonstrated that each instrument has its own sensitivity pattern. Therefore, the selection of lanthanide combinations is a key step in the establishment of a staining panel for mass cytometry‐based experiments, particularly for multicenter studies. We propose the sensitivity pattern as the basis for panel design, instrument standardization and future implementation of normalization algorithms. © 2015 International Society for Advancement of Cytometry
Materialart:
Online-Ressource
ISSN:
1552-4922
,
1552-4930
DOI:
10.1002/cyto.a.22648
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2015
ZDB Id:
2180639-1
SSG:
12
