In:
Cytometry Part B: Clinical Cytometry, Wiley, Vol. 98, No. 2 ( 2020-03), p. 193-202
Abstract:
Several studies have used CD157 in white blood cells with or without proaerolysin (fluorescein‐labeled proaerolysin [FLAER])‐based flow cytometry assays in the diagnosis of paroxysmal nocturnal hemoglobinuria (PNH). Methods We designed a seven‐color CD marker panel comprising FLAER, CD15, CD64, CD24, CD14, CD157, and CD45 to verify CD157's clinical applicability and diagnostic performance in a clinical setting. Results A total of 356 samples were tested. These included 43 PNH‐positive samples and 313 PNH‐negative samples. PNH clones confirmed by the CD157/FLAER combination were almost identical in size to the clones detected by the CD24/CD14/FLAER combination, and the accuracy of the CD157/FLAER combination was 100% in granulocytes and 99.7% in monocytes. Substitution of FLAER with CD157 resulted in 1.9% and 3.5% false‐positives in granulocytes and monocytes, respectively. The accuracy was 98.3% and 96.9% in granulocytes and monocytes, respectively. Moreover, the loss of CD157 expression in granulocytes and monocytes was commonly observed in non‐PNH patients. Some monocytes in non‐PNH patients had weak expression of CD14 but normal expression of FLAER. In this study, PNH clones in granulocytes were always lower than those in matched monocytes. Conclusions We performed the first prospective exploration of the clinical usefulness of FLAER and CD157 in simultaneously recognizing PNH clones in granulocytes and monocytes and verified the applicability of CD157 in substitute for both CD14 and CD24. In the conditions where FLAER is not available, substitution of FLAER with CD157 is acceptable for the identification of PNH clones under the premise of giving full attention to the potential for false‐positives.
Type of Medium:
Online Resource
ISSN:
1552-4949
,
1552-4957
DOI:
10.1002/cyto.b.v98.2
DOI:
10.1002/cyto.b.21847
Language:
English
Publisher:
Wiley
Publication Date:
2020
detail.hit.zdb_id:
2180651-2
SSG:
12
