In:
Developmental Dynamics, Wiley, Vol. 231, No. 4 ( 2004-12), p. 849-858
Kurzfassung:
To evaluate the developmental distribution of adrenergic cells in vivo, we inserted the Cre‐recombinase gene into the locus encoding for the epinephrine biosynthetic enzyme phenylethanolamine n‐methyltransferase (Pnmt) and crossed these Pnmt‐Cre mice with ROSA26 reporter (R26R) mice to activate LacZ (encoding β‐galactosidase) expression in cells that were selectively derived from the adrenergic lineage. Our data show the following: (1) Insertion of Cre‐recombinase into the Pnmt locus created a functional knockout of Pnmt expression with concomitant loss of epinephrine in homozygous Pnmt Cre/Cre mice; (2) Despite the reduction in Pnmt expression and epinephrine production in Pnmt Cre/Cre mice, these mice were viable and fertile, with no apparent developmental defects; (3) When crossed with R26R mice, Pnmt‐Cre activation of LacZ expression faithfully recapitulated Pnmt expression in vivo; and (4) LacZ expression was activated in substantial numbers of pacemaking, conduction, and working cardiomyocytes. Developmental Dynamics 231:849–858, 2004. © 2004 Wiley‐Liss, Inc.
Materialart:
Online-Ressource
ISSN:
1058-8388
,
1097-0177
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2004
ZDB Id:
1473797-8
SSG:
12
