In:
genesis, Wiley, Vol. 50, No. 3 ( 2012-03), p. 316-324
Abstract:
Cell lines are useful tools to facilitate in vitro studies of many biological and molecular processes. We describe a new permanent fibroblast‐type cell line obtained from disaggregated Xenopus tropicalis limb bud. The cell line population doubling time was ∼ 24 h. Its karyotype was genetically stable with a chromosome number of 2 n = 21 and a chromosome 10 trisomy. These cells could be readily transfected and expressed transgenes faithfully. We obtained stable transformants using transposon‐based gene transfer technology. These cells responded to thyroid hormone and thus can provide a complementary research tool to study thyroid hormone signaling events. In conclusion, this cell line baptized “Speedy” should prove useful to couple in vitro and in vivo biological studies in the X. tropicalis frog model. genesis 50:316–324, 2012. © 2011 Wiley Periodicals, Inc.
Type of Medium:
Online Resource
ISSN:
1526-954X
,
1526-968X
Language:
English
Publisher:
Wiley
Publication Date:
2012
detail.hit.zdb_id:
2019664-7
SSG:
12