In:
European Journal of Immunology, Wiley, Vol. 36, No. 12 ( 2006-12), p. 3346-3355
Abstract:
To analyze the effect of vaccine delivery systems on antigen recognition and vaccine efficacy, we compared immune responses in mice immunized either with an adenovirus vector expressing a fusion of Ag85B and ESAT‐6 or with the recombinant fusion protein in a liposomal adjuvant. Both vaccines induced high levels of antigen‐specific IFN‐γ production. The adjuvanted protein vaccine induced primarily a CD4 T cell response directed to the epitope Ag85B 241–255 and gave efficient protection against subsequent Mycobacterium tuberculosis infection. In contrast, the adenoviral construct induced a strong CD8 response predominantly targeted to the epitope ESAT‐6 15–29 and no significant protection against infection. Vaccination with the protein vaccine resulted in highly accelerated recall of Ag85B 241–255 ‐specific T cells immediately post M. tuberculosis challenge whereas the ESAT‐6 15–29 epitope was barely recognized during infection. Delivery of the viral construct in cationic liposomes switched the immune response to a protective one dominated by CD4 T cells targeted to the Ag85B 241–255 epitope. These data demonstrate that the nature of the T cell response to a vaccine antigen is more important than its magnitude with respect to protective efficacy and that vaccine‐mediated changes in immunodominance can result in T cell responses of limited relevance during the natural infection.
Type of Medium:
Online Resource
ISSN:
0014-2980
,
1521-4141
DOI:
10.1002/eji.200636128
Language:
English
Publisher:
Wiley
Publication Date:
2006
detail.hit.zdb_id:
1491907-2
