In:
European Journal of Immunology, Wiley, Vol. 44, No. 5 ( 2014-05), p. 1330-1340
Kurzfassung:
The aryl hydrocarbon receptor ( A h R ) is a ligand‐dependent transcription factor that mediates immunosuppression caused by a variety of environmental contaminants, such as polycyclic aromatic hydrocarbons or dioxins. Recent evidence suggests that A h R plays an important role in T ‐cell‐mediated immune responses by affecting the polarization and differentiation of activated T cells. However, the regulation of A h R expression in activated T cells remains poorly characterized. In the present study, we used purified human T cells stimulated with anti‐ CD 3 and anti‐ CD 28 A bs to investigate the effect of T ‐cell activation on A h R m RNA and protein expression. The expression of A h R m RNA increased significantly and rapidly after T ‐cell activation, identifying A h R as an immediate‐early activation gene. A h R upregulation occurred in all of the T ‐cell subtypes, and is associated with its nuclear translocation and induction of the cytochromes P ‐450 1 A 1 and 1 B 1 m RNA expression in the absence of exogenous signals. In addition, the use of an A h R antagonist or si RNA ‐mediated A h R knockdown significantly inhibited IL ‐22 expression, suggesting that expression and functional activation of A h R is necessary for the secretion of IL ‐22 by activated T cells. In conclusion, our data support the idea that A h R is a major player in T ‐cell physiology.
Materialart:
Online-Ressource
ISSN:
0014-2980
,
1521-4141
DOI:
10.1002/eji.201343920
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2014
ZDB Id:
1491907-2
