In:
European Journal of Immunology, Wiley, Vol. 44, No. 12 ( 2014-12), p. 3532-3542
Abstract:
Even today it is still not completely understood how CD8 + T‐cell memory is maintained long term. Since bone marrow (BM) is a niche for immunological memory, we sought to identify long‐lasting early memory CD8 + T cells in this compartment. To achieve this, we looked for CD8 + T cells that are able to efflux Rhodamine 123, a typical property of stem cells. Indeed, we identified a distinct subset of CD8 + T cells in BM, with the capacity to efflux and high CD127 expression. These CD127 hi effluxers are conventional CD8 + T cells exhibiting a broad TCR‐Vβ repertoire and are generated in response to viral peptides in vitro. CD127 hi effluxer CD8 + T cells have an early memory phenotype defined by preferential TNF‐α production and a Bcl‐2 hi , KLRG‐1 low profile. This population has long telomeres and shows constitutively low frequencies of Ki‐67 expression ex vivo, but has a high proliferative and differentiation capacity in vitro. However, IL‐15 downmodulates CD127 in CD127 hi effluxer CD8 + T cells in vitro. Consequently, the CD127 low effluxer subset may comprise cells recently exposed to IL‐15. Taken together, CD127 hi effluxer CD8 + T cells represent a novel population of early memory T cells resident in BM with properties required for long‐lived memory.
Type of Medium:
Online Resource
ISSN:
0014-2980
,
1521-4141
DOI:
10.1002/eji.201344180
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
1491907-2