In:
ELECTROPHORESIS, Wiley, Vol. 9, No. 7 ( 1988-01), p. 339-343
Abstract:
A method is described which allows to reveal simultaneously the proteolytic patterns of numerous polypeptides separated by two‐dimensional electrophoresis. After two‐dimensional electrophoresis, the gels were dipped successively in buffers for preequilibration, protease digestion, and reequilibration. They were then returned to the electrophoresis tank, and electrophoresis was continued for a short time. After silver staining, digestion products appeared, lined up behind the original polypeptide spots. The method allows proteolytic patterns of numerous polypeptides to be viasualized simply and quickly. Among proteins of wheat leaves, 31 groups of related polypeptides were found according to the similarity of their proteolytic patterns.
Type of Medium:
Online Resource
ISSN:
0173-0835
,
1522-2683
DOI:
10.1002/elps.1150090710
Language:
English
Publisher:
Wiley
Publication Date:
1988
detail.hit.zdb_id:
1475486-1
SSG:
12