In:
ELECTROPHORESIS, Wiley, Vol. 16, No. 1 ( 1995-01), p. 742-743
Abstract:
Somatic mutations in the genes for G‐protein‐coupled receptors which regulate intracellular levels of cyclic AMP have been found in several regions coding for the receptors of melanocyte‐stimulating hormone, adrenaline, luteinizing hormone, rhodopsin and thyrotropin. The mutations found in the thyroid‐stimulating hormone (TSH) receptor are restricted to cells of hyperfunctioning thyroid adenomas and other thyroid tumors. They are thought to lead to a constitutive activation of the receptor independent of TSH. We have developed a temperature‐gradient gel electrophoresis (TGGE) assay starting with the amplification of a part of exon 10 of the TSH‐receptor gene to screen tissue of thyroid tumours. TGGE allows the detection of point mutations even if there are only a few cells with somatic mutations in tissue sections.
Type of Medium:
Online Resource
ISSN:
0173-0835
,
1522-2683
DOI:
10.1002/elps.11501601121
Language:
English
Publisher:
Wiley
Publication Date:
1995
detail.hit.zdb_id:
1475486-1
detail.hit.zdb_id:
619001-7
SSG:
12