In:
ELECTROPHORESIS, Wiley, Vol. 19, No. 16-17 ( 1998-11), p. 3026-3032
Abstract:
The technique of capillary electrophoresis – mass spectrometry (CE‐MS) was applied for determination of isoquinoline alkaloids in crude methanolic extracts of medicinal plants. For the CE separations ammonium formate buffer solutions (70 or 100 m M , pH 3.0 or 4.0) containing 10% methanol or 20–60% acetonitrile as additives were used. The applied voltage was 25 kV, the thermostating temperature was kept constant at 25°C. Coupling with the mass spectrometer was performed via an atmospherical pressure ionization (API) interface and the electrospray ionization technique (ESI). As sheath liquid 5 m M formic acid in acetonitrile at a flow rate of 3 μL/min was used. The spray voltage was 4.5 kV and the temperature of the heated capillary was chosen to be 200°C. Detection in the positive ionization mode resulted in mass spectra showing either the molecular ions [M] + or the protonated molecular ions [M+H] + . The presented method allows detection and identification of isoquinoline alkaloids in crude methanolic extracts of medicinal plants as Eschscholzia californica C HAM . (Papaveraceae), Hydrastis canadensis L. (Ranunculaceae), Berberis vulgaris L. (Berberidaceae), Jateorhiza palmata (L AM .) M IERS (Menispermaceae) and Chelidonium majus L. (Papaveraceae).
Type of Medium:
Online Resource
ISSN:
0173-0835
,
1522-2683
DOI:
10.1002/elps.v19:16/17
DOI:
10.1002/elps.1150191639
Language:
English
Publisher:
Wiley
Publication Date:
1998
detail.hit.zdb_id:
1475486-1
SSG:
12
