In:
ELECTROPHORESIS, Wiley, Vol. 24, No. 15 ( 2003-08), p. 2642-2649
Kurzfassung:
Simultaneous enantioseparations of nine profens for their accurate chiral discrimination were achieved by capillary electrophoresis (CE) in the normal polarity (NP) mode with a single cyclodextrin (CD) system and in the reversed polarity (RP) mode with a dual CD system. The single CD system in the NP mode employed heptakis(2,3,6‐tri‐ O ‐methyl)‐β‐cyclodextrin (TMβCD) added at 75 m M –100 m M 2‐( N ‐morpholino)ethanesulfonic acid buffer (pH 6.0) as the optimum run buffer. The dual CD system operated in the RP mode used 30 m M TMβCD and 1.0% anionic carboxymethyl‐β‐cyclodextrin dissolved in pH 3.0, 100 m M phosphoric acid‐triethanolamine buffer containing 0.01% hexadimethrine bromide added to reverse the electroosmotic flow. Fairly good enantiomeric resolutions and the opposite enantiomer migration orders were achieved in the two modes. Relative migration times to internal standard under respective optimum conditions were characteristic of each enantiomer with good precision ( 〈 2% relative standard deviation, RSD), thereby enabling to crosscheck the chemical identification of profens and also their accurate chiralities. The method linearity in the two modes was found to be adequate ( r ≥ 0.9991) for the chiral assay of the profens investigated. Simultaneous enantiomeric purity test of ibuprofen, ketoprofen and flurbiprofen in a mixture was feasible in a single analysis by the present method.
Materialart:
Online-Ressource
ISSN:
0173-0835
,
1522-2683
DOI:
10.1002/elps.200305450
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2003
ZDB Id:
1475486-1
SSG:
12
