In:
ELECTROPHORESIS, Wiley, Vol. 30, No. 18 ( 2009-09), p. 3242-3249
Abstract:
A study of the influences of embedding artificial structures in a microfluidic device for CE with a free buffer solution is presented. Compared with conventional slab‐gel electrophoresis, three major additional effects on the overall system performance are identified when sub‐micron pillar arrays are integrated into a standard CE microsystem. Since DNA molecules have to migrate in‐between and interact with the pillars, pillar geometry is first demonstrated to have a direct impact on the DNA motion pattern. Electric field re‐distribution is another inevitable outcome when features of sub‐micron dimensions are placed inside a microchannel. This effect is verified by a numerical simulation tool. Furthermore, the integration of the closely packed sub‐micron structures dramatically increases the surface to volume ratios in the microfluidic device and therefore generates a large EOF. The consequence of these additional influences implies a complexity in the measured DNA velocity and indicates that careful considerations have to be taken when these devices are used for DNA electrokinetics study or electrophoresis theory re‐examination.
Type of Medium:
Online Resource
ISSN:
0173-0835
,
1522-2683
DOI:
10.1002/elps.200900127
Language:
English
Publisher:
Wiley
Publication Date:
2009
detail.hit.zdb_id:
1475486-1
SSG:
12
