In:
ELECTROPHORESIS, Wiley, Vol. 31, No. 7 ( 2010-04), p. 1157-1161
Abstract:
The influence of the BGE composition, including the addition of a single‐isomer sulfated β‐CD derivative, on the ionization performance of the model compound carvedilol in NACE‐ESI‐MS was studied using an alternative infusion method. This approach employs voltage‐induced infusion of the BGE containing the analyte, and takes into account the effects of variations in EOF and effective analyte mobility on the ESI‐MS intensity. First, the optimal composition of the sheath liquid for CE‐MS in terms of signal abundance and stability was determined. The BGE ammonium formate, acetate, and camphorsulfonate were found to have similar effects on analyte ionization. Addition of single‐isomer sulfated β‐CD derivatives (available as sodium salt) to the BGE revealed that the anionic CD derivatives did not give rise to the same ionization suppression effect. This result can be attributed to differences in the dissociation state of these sodium salts. Finally, it is shown that information about chiral selectivity can also be obtained with the applied infusion method.
Type of Medium:
Online Resource
ISSN:
0173-0835
,
1522-2683
DOI:
10.1002/elps.200900649
Language:
English
Publisher:
Wiley
Publication Date:
2010
detail.hit.zdb_id:
1475486-1
SSG:
12
