In:
ELECTROPHORESIS, Wiley, Vol. 32, No. 24 ( 2011-12), p. 3600-3611
Abstract:
Epithelial tissue lining the inner side of the urinary bladder is the most common target for bladder cancer‐related diseases. Bladders of freshly slaughtered pigs were utilised for a comprehensive analysis of the proteome and phosphoproteome of bladder epithelial cells. Following protein separation by 2‐D gel electrophoresis and identification by matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometry (MALDI‐TOF‐MS) the first proteome and phosphoproteome maps of pig urinary bladder epithelial cells (PUBEC) were established. A total of 120 selected protein spots were identified. By using the La 3+ enrichment method further developed in our laboratory we identified 31 phosphoproteins with minimal contamination by non‐phosphopeptides. The 2‐DE map of pig urothelial cells may prove as a useful tool for studies on uroepithelial biology, and the analysed phosphoproteins expression pattern, together with the whole cell proteome, will be helpful for identifying the proteins involved in bladder‐related diseases.
Type of Medium:
Online Resource
ISSN:
0173-0835
,
1522-2683
DOI:
10.1002/elps.201100220
Language:
English
Publisher:
Wiley
Publication Date:
2011
detail.hit.zdb_id:
1475486-1
SSG:
12