In:
ELECTROPHORESIS, Wiley, Vol. 33, No. 18 ( 2012-09), p. 2903-2910
Kurzfassung:
Photolytic optical gating ( POG ) facilitates rapid, on‐line and highly sensitive analyses, though POG utilizes UV lasers for sample injection. We present a low‐cost, more portable alternative, employing an ultraviolet light‐emitting diode (UV‐LED) array to inject caged fluorescent dyes via photolysis. Utilizing the UV ‐ LED array, labeled amino acids were injected with nanomolar limits of detection (270 ± 30 nM and 250 ± 30 nM for arginine and citrulline, respectively). When normalized for the difference in light intensity, the UV ‐ LED array provides comparable sensitivity to POG utilizing UV lasers. Additionally, the UV ‐ LED array yielded sufficient beam quality and stability to facilitate coupling with a Hadamard transform, resulting in increased sensitivity. This work shows, for the first time, the use of an UV ‐ LED for online POG with comparable sensitivity to conventional laser sources but at a lower cost.
Materialart:
Online-Ressource
ISSN:
0173-0835
,
1522-2683
DOI:
10.1002/elps.201200279
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2012
ZDB Id:
1475486-1
SSG:
12