In:
ELECTROPHORESIS, Wiley, Vol. 34, No. 13 ( 2013-07), p. 1931-1938
Kurzfassung:
We developed the dual‐micropillar‐based microfluidic platform to direct embryonic stem (ES) cell fate. 4 × 4 dual‐micropillar‐based microfluidic platform consisted of 16 circular‐shaped outer micropillars and 8 saddle‐shaped inner micropillars in which single ES cells were cultured. We hypothesized that dual‐micropillar arrays would play an important role in controlling the shear stress and cell docking. Circular‐shaped outer micropillars minimized the shear stress, whereas saddle‐shaped inner micropillars allowed for docking of individual ES cells. We observed the effect of saddle‐shaped inner micropillars on cell docking in response to hydrodynamic resistance. We also demonstrated that ES cells cultured for 6 days within the dual‐micropillar‐based microfluidic platform differentiated into neural‐like cells. Therefore, this dual‐micropillar‐based microfluidic platform could be a potentially powerful method for screening of lineage commitments of single ES cells.
Materialart:
Online-Ressource
ISSN:
0173-0835
,
1522-2683
DOI:
10.1002/elps.201200578
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2013
ZDB Id:
1475486-1
SSG:
12
