In:
ELECTROPHORESIS, Wiley, Vol. 39, No. 3 ( 2018-02), p. 496-503
Abstract:
Combining high‐resolution imaging and electrophysiological recordings is key for various types of experimentation on lipid bilayers and ion channels. Here, we propose an integrated biosensing platform consisting of a microfluidic cartridge and a dedicated chip‐holder to conduct such dual measurements on suspended lipid bilayers, in a user‐friendly manner. To illustrate the potential of the integrated platform, we characterize lipid bilayers in terms of thickness and fluidity while simultaneously monitoring single ion channel currents. For that purpose, POPC lipid bilayers are supplemented with a fluorescently‐tagged phospholipid (NBD‐PE, 1% mol) for Fluorescence Recovery After Photobleaching (FRAP) measurements and a model ion channel (gramicidin, 1 nM). These combined measurements reveal that NBD‐PE has no effect on the lipid bilayer thickness while gramicidin induces thinning of the membrane. Furthermore, the presence of gramicidin does not alter the lipid bilayer fluidity. Surprisingly, in lipid bilayers supplemented with both probes, a reduction in gramicidin open probability and lifetime is observed compared to lipid bilayers with gramicidin only, suggesting an influence of NBD‐PE on the gramicidin ion function. Altogether, our proposed microfluidic biosensing platform in combination with the herein presented multi‐parametric measurement scheme paves the way to explore the interdependent relationship between lipid bilayer properties and ion channel function.
Type of Medium:
Online Resource
ISSN:
0173-0835
,
1522-2683
DOI:
10.1002/elps.201700346
Language:
English
Publisher:
Wiley
Publication Date:
2018
detail.hit.zdb_id:
1475486-1
SSG:
12
