In:
Journal of Applied Toxicology, Wiley, Vol. 31, No. 5 ( 2011-07), p. 455-462
Abstract:
Genomic analysis in the local lymph node assays (LLNAs) is useful for assessing skin sensitization of chemicals and providing insights into mechanisms of sensitization. In this study, we collected 1406 genes from previous microarray findings, validated changes in their expression by RT‐PCR analysis in local lymph nodes draining skin exposed to different sensitizers, and interpreted their biological function through pathway‐based genomic analysis, in which 468 genes were identified as being in the KEGG pathway database. The top‐ranked functions ( P 〈 0.01) identified as being affected by the sensitizers were associated with aspects of cell growth, such as DNA replication, cell cycle regulation and pyrimidine metabolism. All the sensitizers tested (DNCB, OXA and TDI) induced significant up‐regulation of Psme4 , Tfdp1 and Dut identified in these functional categories. Specific changes were also shown in functional categories related to the immune response, such as Ccl21c , Cxcl9 , Cxcl10 , Ifng and Il12rb1 , which were found to have functional relevance. These findings may enhance our understanding and assessment of chemical sensitizers, and enable us to distinguish sensitizers from irritants and to classify chemicals as contact sensitizers.
Type of Medium:
Online Resource
ISSN:
0260-437X
,
1099-1263
Language:
English
Publisher:
Wiley
Publication Date:
2011
detail.hit.zdb_id:
1475015-6
