In:
Journal of Biophotonics, Wiley, Vol. 7, No. 9 ( 2014-09), p. 703-712
Abstract:
Raman photon generation inside human skin and escaping to skin surface were modeled in an eight‐layered skin optical model. Intrinsic Raman spectra of different skin layers were determined by microscopy measurements of excised skin tissue sections. Monte Carlo simulation was used to study the excitation light distribution and intrinsic Raman signal distortion caused by tissue reabsorption and scattering during in vivo measurements. The simulation results demonstrated how different skin layers contributed to the observed in vivo Raman spectrum. Using the strongest Raman peak at 1445 cm –1 as an example, the simulation suggested that the integrated contributions of the stratum corneum layer is 1.3%, the epidermis layer 28%, the dermis layer 70%, and the subcutaneous fat layer 1.1%. Reasonably good matching between the calculated spectrum and the measured in vivo Raman spectra was achieved, thus demonstrated great utility of our modeling method and approaches for help understanding the clinical measurements. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Type of Medium:
Online Resource
ISSN:
1864-063X
,
1864-0648
DOI:
10.1002/jbio.201300045
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2403788-6
