In:
Microscopy Research and Technique, Wiley, Vol. 79, No. 4 ( 2016-04), p. 298-303
Abstract:
The preparation of thinned lamellae from bulk samples for transmission electron microscopy (TEM) analysis has been possible in the focussed ion beam scanning electron microscope (FIB‐SEM) for over 20 years via the in situ lift‐out method. Lift‐out offers a fast and site specific preparation method for TEM analysis, typically in the field of materials science. More recently it has been applied to a low‐water content biological sample (Rubino 2012). This work presents the successful lift‐out of high‐water content lamellae, under cryogenic conditions (cryo‐FIB lift‐out) and using a nanomanipulator retaining its full range of motion, which are advances on the work previously done by Rubino (2012). Strategies are explored for maintaining cryogenic conditions, grid attachment using cryo‐condensation of water and protection of the lamella when transferring to the TEM. Microsc. Res. Tech. 79:298–303, 2016 . © 2016 Wiley Periodicals, Inc.
Type of Medium:
Online Resource
ISSN:
1059-910X
,
1097-0029
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
1474912-9
SSG:
11
SSG:
12