In:
Microscopy Research and Technique, Wiley, Vol. 83, No. 4 ( 2020-04), p. 332-337
Abstract:
The aim of this study was to evaluate the toxicity of titanium dioxide nanoparticles (TiO 2 NPs) by short‐term toxicity tests in Apis mellifera , considered an excellent bioindicator organism mainly due to its sensitivity. Bees have been exposed to several concentrations of TiO 2 NPs (1 × 10 −3 , 1 × 10 −4 , 1 × 10 −5 , 1 × 10 −6 mg/10 ml) for 10 days. Morphostructural and histological assays were done on gut and honey sac. The research of exposure biomarkers like metallothioneins 1 (MT1) and Heat Shock Protein 70 (HSP70) was performed to verify if a detoxification mechanism has been activated in the exposed animals. No histological alteration on the epithelium of the gut and honey sac were observed in exposed samples. A significant positivity for anti‐MT1 antibody was observed only in the honey sac cells. A weak positivity for HSP70 was observed in both structures analyzed. In several studies have shown the non‐toxicity of TiO 2 NPs on other model organisms, in our study, titanium dioxide nanoparticles was proven to be highly toxic at the highest concentration tested (100% of lethality to 1 × 10 −3 mg/10 ml) and moderately toxic at lower concentrations. Honey bees proved to be excellent models for study of NPs toxicity and for monitoring environment.
Type of Medium:
Online Resource
ISSN:
1059-910X
,
1097-0029
Language:
English
Publisher:
Wiley
Publication Date:
2020
detail.hit.zdb_id:
1474912-9
SSG:
11
SSG:
12