In:
The Journal of Gene Medicine, Wiley, Vol. 10, No. 7 ( 2008-07), p. 810-820
Kurzfassung:
Autoimmune ovarian disease (AOD) caused by auto‐reactive T cells is considered a major reason for human premature ovarian failure, which affects 5% of women worldwide. Methods and Results To develop an effective treatment for AOD, we showed that the co‐administration of mouse zona pellucida protein 3 (mZP3) protein and DNA vaccine encoding the mZP3 was able to meliorate AOD in an AOD murine model induced by the mZP3. We observed that established AOD in mice reverted to a normal ovarian morphology without notable T‐cell infiltration in the co‐administrated group; whereas mice in the control groups developed severe AOD. The amelioration appears to be antigen specific because other co‐administration combinations failed to reverse AOD and correlates with significant reductions of pathogenic T‐cell responses and productions of tumor necrosis factor‐α and interferon‐γ. Furthermore, the melioration is apparently associated with the induction of mZP3 specific regulatory T cells that exhibit a phenotypic CD4 + CD25 − FoxP3 + IL‐10 + in the co‐administrated group, which can be transferred to reverse AOD in vivo . Conclusions Thus, co‐administration of mZP3 DNA and protein vaccines can be used to treat established AOD, and may provide a novel immunotherapy strategy to treat other autoimmune diseases. Copyright © 2008 John Wiley & Sons, Ltd.
Materialart:
Online-Ressource
ISSN:
1099-498X
,
1521-2254
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2008
ZDB Id:
2002203-7
SSG:
12
