In:
Journal of Leukocyte Biology, Oxford University Press (OUP), Vol. 38, No. 2 ( 1985-08-01), p. 213-230
Abstract:
A rapid method for mass isolation of functionally intact hepatocytes and reticuloendothelial cells from a single rat liver is described. The technique is based on collagenase perfusion of the liver, isopycnic sedimentation in Percoll, and selective adherence of the cells. The Kupffer cells (KC) attach and spread on glass or plastic in serum-free medium 15 min following seeding. Cultures of KC are 90%–95% pure with about 5% liver endothelial cells (LEC), & lt;1% parenchymal cells (PC) and a maximum of 5% stellate cells (SC). The LEC adhere and spread on fibronectin 60–120 min following seeding, forming cultures that are contaminated with 5–10% SC and & lt;1% KC and PC. The yield of plated LEC is 50–60 × 106 per 200-g rat. Ultrastructural analysis shows that Percoll does not associate with the cells during the separation procedure.
Type of Medium:
Online Resource
ISSN:
0741-5400
,
1938-3673
DOI:
10.1002/jlb.38.2.213
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
1985
detail.hit.zdb_id:
2026833-6
SSG:
12