In:
Journal of Mass Spectrometry, Wiley, Vol. 38, No. 3 ( 2003-03), p. 296-304
Abstract:
Mass correlated acceleration (MCA) has now been integrated into a 4 in (10.2 cm) matrix‐assisted laser desorption/ionization time‐of‐flight (MALDI‐TOF) mass spectrometer to achieve high resolving power across a broader mass range, without sacrificing detection of higher mass ions. The goal was to combine MCA with a custom‐built miniaturized instrument such as those that might be used for field‐portable applications. Unlike other pulsed extraction methods, MCA is not mass dependent and mass spectra can be achieved with a single tuning of instrument parameters. Additionally, the multi‐channel recording advantage is better realized because ions of all masses can be brought into focus simultaneously. The MCA dual‐stage ion source compensates dynamically for the mass dependence by incorporating an extraction pulse region followed by an acceleration region that contains a time‐dependent waveform correlated with mass. The technique was validated with applications in peptide mixtures and protein digestions. Diagnostic studies for the instrument include m / z range and limits of detection. Copyright © 2003 John Wiley & Sons, Ltd.
Type of Medium:
Online Resource
ISSN:
1076-5174
,
1096-9888
Language:
English
Publisher:
Wiley
Publication Date:
2003
detail.hit.zdb_id:
2197367-2
detail.hit.zdb_id:
1472468-6
detail.hit.zdb_id:
7414-7
SSG:
11
SSG:
12
