In:
Journal of Orthopaedic Research, Wiley, Vol. 32, No. 6 ( 2014-06), p. 834-841
Kurzfassung:
This study evaluated the effect of local zinc chloride (ZnCl 2 ), an insulin mimetic agent, upon the early and late parameters of fracture healing in rats using a standard femur fracture model. Mechanical testing, radiographic scoring, histomorphometry, qualitative histological scoring, PCNA immunohistochemistry, and local growth factor analysis were performed. Fractures treated with local ZnCl 2 possessed significantly increased mechanical properties compared to controls at 4 weeks post fracture. The radiographic scoring analysis showed increased cortical bridging at 4 weeks in the 1.0 ( p = 0.0015) and 3.0 ( p 〈 0.0001) mg/kg ZnCl 2 treated groups. Histomorphometry of the fracture callus at day 7 showed 177% increase ( p = 0.036) in percent cartilage and 133% increase ( p = 0.002) in percent mineralized tissue with local ZnCl 2 treatment compared to controls. Qualitative histological scoring showed a 2.1× higher value at day 7 in the ZnCl 2 treated group compared to control ( p = 0.004). Cell proliferation and growth factors, VEGF and IGF‐I, within fracture calluses treated with local ZnCl 2 were increased at day 7. The results suggest local administration of ZnCl 2 increases cell proliferation, causing increased growth factor production which yields improved chondrogenesis and endochondral ossification. Ultimately, these events lead to accelerated fracture healing as early as 4 weeks post fracture. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:834–841, 2014.
Materialart:
Online-Ressource
ISSN:
0736-0266
,
1554-527X
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2014
ZDB Id:
2050452-4
