In:
Journal of Separation Science, Wiley, Vol. 34, No. 16-17 ( 2011-08), p. 2072-2078
Abstract:
A simple and sensitive method has been developed for the simultaneous determination of polar nonsteroidal pharmaceuticals and metabolites, including acetaminophen, p ‐aminophenol and several aspirin metabolites (salicylic acid, gentisic acid, salicyluric acid and 2,3‐dihydroxybenzoic acid), by capillary liquid chromatography with amperometric detection. Using a capillary monolithic column with mixed mode stationary phases and a mobile phase composed of acetonitrile and Tris buffer, rapid separation of six polar analytes was achieved within 8 min, and a hydrophilic interaction and strong anion exchange separation mechanism were exhibited. Method detection limits of six analytes ranged from 10 to 50 ng/mL. In terms of precision, the intra‐ and interday relative standard deviation values in all analytes never exceeded 3.1% for migration time and 8.9% for peak areas, respectively. This method provided a simple, rapid and cost‐effective approach for the analysis of polar pharmaceuticals. The applicability of the method in pharmacokinetics was verified by spiking human serum samples with the compounds and analyzing the recoveries.
Type of Medium:
Online Resource
ISSN:
1615-9306
,
1615-9314
DOI:
10.1002/jssc.v34.16/17
DOI:
10.1002/jssc.201100163
Language:
English
Publisher:
Wiley
Publication Date:
2011
detail.hit.zdb_id:
2047990-6