In:
The Laryngoscope, Wiley, Vol. 123, No. 2 ( 2013-02), p. 315-320
Kurzfassung:
The aim of this study was to investigate the effects of a 1.8 GHz continuous electromagnetic fields (EMF) on human nasal mucociliary transport, and to determine the pathophysiology of ciliary beat frequency (CBF) during an EMF‐induced change. Methods: Human nasal mucosa cells were exposed to a 1.8 GHz EMF (SAR=1.0 W/kg), and CBF was analyzed using an optical flow technique with the peak detection method. Results: The 1.8 GHz‐exposed group showed a decreased CBF when compared to the control group. In the cytotoxicity assay, difference in survival rates was not found between the two groups. In the EMF‐exposed group, protein kinase C (PKC) activity was increased during a PKC activity assay. The broad PKC inhibitor, Calphostin C abolished the EMF‐induced decrease of CBF. The EMF‐induced decrease of CBF was abolished by GF 109203X, a novel PKC (nPKC) isoform inhibitor, whereas the decrease was not attenuated by Gö‐6976, a specific inhibitor of conventional PKC (cPKC) isoform. Conclusions: EMF may inhibit CBF via an nPKC‐dependent mechanism. Therefore, we have confirmed that EMF could decrease CBF by increasing PKC activity. Laryngoscope, 2012
Materialart:
Online-Ressource
ISSN:
0023-852X
,
1531-4995
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2013
ZDB Id:
2026089-1