In:
Macromolecular Bioscience, Wiley, Vol. 8, No. 12 ( 2008-12-08), p. 1126-1134
Abstract:
Surface properties have a significant influence on the performance of biomedical devices. The influence of surface chemistry on the amount and distribution of adsorbed proteins has been evaluated by a combination of atomic force microscopy (AFM) and surface plasmon resonance (SPR). Adsorption of albumin, fibrinogen, and fibronectin was analyzed under static and dynamic conditions, employing self‐assembled monolayers (SAMs) as model surfaces. AFM was performed in tapping mode with antibody‐modified tips. Phase‐contrast images showed protein distribution on SAMs and phase‐shift entity provided information on protein conformation. SPR analysis revealed substrate‐specific dynamics in each system investigated. When multi‐protein solutions and diluted human plasma interacted with SAMs, SPR data suggested that surface chemistry governs the equilibrium composition of the protein layer. magnified image
Type of Medium:
Online Resource
ISSN:
1616-5187
,
1616-5195
DOI:
10.1002/mabi.200800110
Language:
English
Publisher:
Wiley
Publication Date:
2008
detail.hit.zdb_id:
2039130-4
SSG:
12