In:
mLife, Wiley, Vol. 1, No. 4 ( 2022-12), p. 448-459
Abstract:
In this work, we developed an automatic and index‐based system called EasySort AUTO for single‐cell identification and sorting of microbial cells. Under a positive‐mount microscope, target single cells are identified via image‐based artificial intelligence algorithms and then moved from cell populations via optical tweezers, followed by exportation to PCR tubes through an automatic collection platform. During this process, a single cell is packaged in a microdroplet and automatically exported in a precisely indexed, “One‐Cell‐One‐Tube” manner. As it is automated, index‐based, and vitality preserved, the EasySort AUTO system can find broad applications in supplying target single cells from microbiome samples for single‐cell sequencing or cultivation.
Type of Medium:
Online Resource
ISSN:
2770-100X
,
2770-100X
Language:
English
Publisher:
Wiley
Publication Date:
2022
detail.hit.zdb_id:
3120417-X