In:
Molecular Nutrition & Food Research, Wiley, Vol. 61, No. 10 ( 2017-10)
Abstract:
Recent reports indicate that gut microbiota and their metabolites may regulate host inflammatory conditions, including the chronic inflammation of obese adipose tissues. In this study, we investigated whether specific synthesized fatty acids, identical to the metabolites generated by gut microbiota, act as anti‐inflammatory factors in obesity‐induced inflammation. Methods and results We first used lipopolysaccharide (LPS)‐stimulated RAW 264.7 macrophages to examine the anti‐inflammatory effect of fatty acids synthesized to resemble representative polyunsaturated fatty acid metabolites from gut microbiota. Fatty acids containing an enone structure showed the most potent anti‐inflammatory activity. Enone fatty acids also displayed anti‐inflammatory effects on macrophages cocultured with hypertrophied 3T3‐L1 or immortalized primary adipocytes; and macrophages stimulated with 3T3‐L1 adipocyte conditioned medium. Consistently, the beneficial outcome was revealed in the case of LPS‐ and obesity‐induced inflammatory cytokine stimulation in ex vivo adipose tissues. Furthermore, these fatty acids recovered the suppression of β‐adrenergic receptor‐stimulated uncoupling protein 1 expression and secretion of adiponectin in C3H10T1/2 and 3T3‐L1 adipocytes, respectively, under inflammatory conditions, suggesting that enone fatty acids can ameliorate dysfunctions of adipocytes induced by inflammation. Conclusion These findings indicate that synthesized enone fatty acids show potent anti‐inflammatory effects, leading to the improvement of inflammation‐induced dysfunctions in adipocytes.
Type of Medium:
Online Resource
ISSN:
1613-4125
,
1613-4133
DOI:
10.1002/mnfr.201700064
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
2160372-8
SSG:
12