In:
The Journal of Pathology, Wiley, Vol. 222, No. 1 ( 2010-09), p. 76-88
Abstract:
As a member of the catenin family, little is known about the clinical significance and possible mechanism of δ‐catenin expression in numerous tumours. We examined the expression of δ‐catenin by immunohistochemistry in 115 cases of non‐small cell lung cancer (NSCLC) (including 65 cases with follow‐up records and 50 cases with paired lymph node metastasis lesions). The mRNA and protein expression of δ‐catenin was also detected in 30 cases of paired lung cancer tissues and normal lung tissues by RT‐PCR and western blotting, respectively. Co‐immunoprecipitation was used to examine whether δ‐catenin competitively bound to E‐cadherin with p120ctn in lung cancer cells or not. The effects of δ‐catenin on the activity of small GTPases and the biological behaviour of lung cancer cells were explored by pull‐down assay, flow cytometry, MTT, and Matrigel invasive assay. The results showed that the mRNA and protein expression of δ‐catenin was increased in lung cancer tissues; the positive expression rate of δ‐catenin was significantly increased in adenocarcinoma, stage III–IV, paired lymph node metastasis lesions, and primary tumours with lymph node metastasis (all p 〈 0.05); and the postoperative survival period of patients with δ‐catenin‐positive expression was shorter than that of patients with δ‐catenin‐negative expression ( p 〈 0.05). No competition between δ‐catenin and p120ctn for binding to E‐cadherin in cytoplasm was found in two lung cancer cell lines. By regulating the activity of small GTPases and changing the cell cycle, δ‐catenin could promote the proliferation and invasion of lung cancer cells. We conclude that δ‐catenin is an oncoprotein overexpressed in NSCLC and that increased δ‐catenin expression is critical for maintenance of the malignant phenotype of lung cancer. Copyright © 2010 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Type of Medium:
Online Resource
ISSN:
0022-3417
,
1096-9896
Language:
English
Publisher:
Wiley
Publication Date:
2010
detail.hit.zdb_id:
1475280-3
