In:
Plant Direct, Wiley, Vol. 1, No. 4 ( 2017-10)
Abstract:
Transcription factors ( TF s) regulate the expression of other genes to indirectly mediate stress resistance mechanisms. Therefore, when studying TF ‐mediated stress resistance, it is important to understand how TF s interact with genes in the genetic background. Here, we fine‐mapped the aluminum (Al) resistance QTL Alt12.1 to a 44‐kb region containing six genes. Among them is ART 1 , which encodes a C2H2‐type zinc finger TF required for Al resistance in rice. The mapping parents, Al‐resistant cv Azucena ( tropical japonica ) and Al‐sensitive cv IR 64 ( indica ), have extensive sequence polymorphism within the ART 1 coding region, but similar ART 1 expression levels. Using reciprocal near‐isogenic lines ( NIL s) we examined how allele‐swapping the Alt12.1 locus would affect plant responses to Al. Analysis of global transcriptional responses to Al stress in roots of the NIL s alongside their recurrent parents demonstrated that the presence of the Alt12.1 from Al‐resistant Azucena led to greater changes in gene expression in response to Al when compared to the Alt12.1 from IR 64 in both genetic backgrounds. The presence of the ART 1 allele from the opposite parent affected the expression of several genes not previously implicated in rice Al tolerance. We highlight examples where putatively functional variation in cis ‐regulatory regions of ART 1‐regulated genes interacts with ART 1 to determine gene expression in response to Al. This ART 1–promoter interaction may be associated with transgressive variation for Al resistance in the Azucena × IR 64 population. These results illustrate how ART 1 interacts with the genetic background to contribute to quantitative phenotypic variation in rice Al resistance.
Type of Medium:
Online Resource
ISSN:
2475-4455
,
2475-4455
DOI:
10.1002/pld3.2017.1.issue-4
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
2912669-1