In:
PROTEOMICS, Wiley, Vol. 21, No. 3-4 ( 2021-02)
Abstract:
Single‐cell RNA sequencing on circulating tumor cells (CTCs) proves useful to study mechanisms of tumor heterogeneity, metastasis, and drug resistance. Currently, single‐cell RNA sequencing of CTCs usually takes three prerequisite steps: enrichment of CTCs from whole blood, characterization of captured cells by immunostaining and microscopic imaging, and single‐cell isolation through micromanipulation. However, multiple pipetting and transferring steps can easily cause the loss of rare CTCs. To address this issue, a novel integrated microfluidic chip for sequential enrichment, isolation, and characterization of CTCs at single‐cell level, is developed. And, single CTC lysis is achieved on the same chip. The microfluidic chip includes functions of blood clot filtration, single‐cell isolation, identification, and target single‐cell lysate collection. By spiking tumor cells into whole blood, it is validated that this microfluidic chip can effectively conduct single‐cell CTCs RNA sequencing. The approach lays a solid foundation for the analysis of RNA expression profiling of single‐cell CTCs.
Type of Medium:
Online Resource
ISSN:
1615-9853
,
1615-9861
DOI:
10.1002/pmic.v21.3-4
DOI:
10.1002/pmic.202000060
Language:
English
Publisher:
Wiley
Publication Date:
2021
detail.hit.zdb_id:
2037674-1
SSG:
12