In:
Proteins: Structure, Function, and Bioinformatics, Wiley, Vol. 49, No. 4 ( 2002-12), p. 543-553
Abstract:
Interleukin enhancer binding factor (ILF) binds to the interleukin‐2 (IL‐2) promoter and regulates IL‐2 gene expression. In this study, the 3D structure of the DNA‐binding domain of ILF was determined by multidimensional NMR spectroscopy. NMR structure analysis revealed that the DNA‐binding domain of ILF is a new member of the winged helix/forkhead family, and that its wing 2 contains an extra α‐helix. This is the first study to report the presence of a C‐terminal α‐helix in place of a typical wing 2 in a member of this family. This structural difference may be responsible for the different DNA‐binding specificity of ILF compared to other winged helix/forkhead proteins. Our deletion studies of the fragments of ILF also suggest that the C‐terminal region plays a regulatory role in DNA binding. Proteins 2002;49:543–553. © 2002 Wiley‐Liss, Inc.
Type of Medium:
Online Resource
ISSN:
0887-3585
,
1097-0134
Language:
English
Publisher:
Wiley
Publication Date:
2002
detail.hit.zdb_id:
1475032-6
SSG:
12