In:
Stress and Health, Wiley, Vol. 27, No. 3 ( 2011-08)
Abstract:
Stress is a defense mechanism of an organism and is directly related to homeostasis, which is the equilibrium state of the multitude of organism systems, both among each other and with the environment. Given that stress is a major cause of cellular instability, it is directly related to its loss homeostasis. The present study assessed the changes caused by stress on the genetic material of Rattus norvegicus using micronuclei analysis. A total of 10 males were studied by being submitted to continuous stress from photoperiod, temperature and noise for 26 days. Peripheral blood samples were obtained immediately before submitting the animals to stress, as well as on the fifteenth and twenty‐sixth days afterwards. Blood smears were stained with Giemsa and the slides were analyzed using light microscopy. Approximately 3,000 polychromatic erythrocytes from each animal were observed and classified as either normal or micronucleated. The analysis indicated a frequency of 0.0012014 for the control sample, whereas samples on days 15 and 26 had frequencies of 0.0035458 and 0.0496850, respectively. A statistical analysis revealed statistically significant differences among the studied cell samples (ANOVA p 〈 0.00001, significance of 5%). Therefore, the presence of micronuclei in the studied samples is consistent with a cause/effect relationship. Copyright © 2010 John Wiley & Sons, Ltd.
Type of Medium:
Online Resource
ISSN:
1532-3005
,
1532-2998
Language:
English
Publisher:
Wiley
Publication Date:
2011
detail.hit.zdb_id:
2054572-1
