In:
Stem Cells, Oxford University Press (OUP), Vol. 37, No. 8 ( 2019-08-01), p. 1018-1029
Kurzfassung:
The transcription factor Oct4 plays a key regulatory role in the induction and maintenance of cellular pluripotency. In this article, we show that ubiquitous and multifunctional poly(C) DNA/RNA-binding protein hnRNP-K occupies Oct4 (Pou5f1) enhancers in embryonic stem cells (ESCs) but is dispensable for the initiation, maintenance, and downregulation of Oct4 gene expression. Nevertheless, hnRNP-K has an essential cell-autonomous function in ESCs to maintain their proliferation and viability. To better understand mechanisms of hnRNP-K action in ESCs, we have performed ChIP-seq analysis of genome-wide binding of hnRNP-K and identified several thousands of hnRNP-K target sites that are frequently co-occupied by pluripotency-related and common factors (Oct4, TATA-box binding protein, Sox2, Nanog, Otx2, etc.), as well as active histone marks. Furthermore, hnRNP-K localizes exclusively within open chromatin, implying its role in the onset and/or maintenance of this chromatin state. Stem Cells 2019;37:1018–1029
Materialart:
Online-Ressource
ISSN:
1066-5099
,
1549-4918
Sprache:
Englisch
Verlag:
Oxford University Press (OUP)
Publikationsdatum:
2019
ZDB Id:
2030643-X
ZDB Id:
1143556-2
ZDB Id:
605570-9
SSG:
12
