In:
Bioscience Reports, Portland Press Ltd., Vol. 11, No. 3 ( 1991-06-01), p. 139-145
Abstract:
High-affinity binding of3H-folate in Triton X-100 solubilized membranes of human liver displayed characteristics, e.g. apparent positive cooperativity, which are typical of specific folate binding. Ultrogel® AcA 44 chromatography of solubilized membranes saturated with3H-folate revealed a major peak of 100 kDa and a minor peak of 25 kDa. The 100 kDa peak could represent a hydrophobic membrane associated molecular form of the protein. This notion was supported by the fact that the two peaks had identical molecular weights as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis with immunoblotting.
Type of Medium:
Online Resource
ISSN:
0144-8463
,
1573-4935
Language:
English
Publisher:
Portland Press Ltd.
Publication Date:
1991
detail.hit.zdb_id:
2014993-1
SSG:
12