In:
Microchimica Acta, Springer Science and Business Media LLC, Vol. 189, No. 2 ( 2022-02)
Abstract:
The possibility to prepare molecularly imprinted nanoparticles from silk fibroin was recently demonstrated starting from methacrylated silk fibroin and choosing a protein as template. Here, we attempted the imprinting of fibroin-based molecularly imprinted polymers (MIPs), called bioMIPs, using as a template hepcidin that is a iron-metabolism regulator-peptide, possessing a hairpin structure. A homogeneous population (PDI 〈 0.2) of bioMIPs with size ~50 nm was produced. The bioMIPs were selective for the template; the estimated dissociation constant for hepcidin was K D = 3.6 ± 0.5 10 −7 M and the average number of binding sites per bioMIP was equal to 2. The bioMIPs used in a competitive assay for hepcidin in serum showed a detection range of 1.01 10 −7 – 6.82 10 −7 M and a limit of detection of 3.29 10 −8 M. Graphical abstract
Type of Medium:
Online Resource
ISSN:
0026-3672
,
1436-5073
DOI:
10.1007/s00604-022-05165-0
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2022
detail.hit.zdb_id:
1462152-6
