In:
European Journal of Clinical Microbiology & Infectious Diseases, Springer Science and Business Media LLC, Vol. 40, No. 8 ( 2021-08), p. 1609-1622
Abstract:
This study compared standard of care testing (SOC) to BioFire® FilmArray® Pneumonia plus Panel (PN plus ). PN plus detects 15 bacteria with semiquantitative log bin values, 7 antibiotic resistance markers, three atypical bacteria (AB), and eight viral classes directly from bronchoalveolar lavage-like specimens (BLS) and sputum-like specimens (SLS). Fifty-two laboratories from 13 European countries and Israel tested 1234 BLS and 1242 SLS with PN plus and SOC. Detection rates and number of pathogens/samples were compared for PN plus pathogens. PN plus bin values and SOC quantities were compared. Three thousand two hundred sixty-two bacteria in PN plus were detected by PN plus and/or SOC. SOC detected 57.1% compared to 95.8% for PN plus ( p ≤ 0.0001). PN plus semiquantitative bin values were less than SOC, equal to SOC, or greater than SOC in 5.1%, 25.4%, and 69.6% of results, respectively. PN plus bin values were on average ≥ 1 log than SOC values (58.5% 1–2 logs; 11.0% 3–4 logs). PN plus identified 98.2% of MRSA and SOC 55.6%. SOC detected 73/103 AB (70.9%) and 134/631 viruses (21.2%). PN plus detected 93/103 AB (90.3%) and 618/631 viruses (97.9%) ( p ≤ 0.0001). PN plus and SOC mean number of pathogens/samples were 1.99 and 1.44, respectively. All gram-negative resistance markers were detected. PN plus and SOC results were fully or partially concordant for 49.1% and 26.4% of specimens, respectively. PN plus was highly sensitive and detected more potential pneumonia pathogens than SOC. Semiquantification may assist in understanding pathogen significance. As PN plus generates results in approximately 1 h, PN plus has potential to direct antimicrobial therapy in near real time and improve antimicrobial stewardship and patient outcomes.
Type of Medium:
Online Resource
ISSN:
0934-9723
,
1435-4373
DOI:
10.1007/s10096-021-04195-5
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2021
detail.hit.zdb_id:
1459049-9
