In:
Biotechnology Letters, Springer Science and Business Media LLC, Vol. 42, No. 1 ( 2020-01), p. 115-124
Abstract:
To thoroughly characterize the P ylb promoter and identify the elements that affect the promoter activity. Result The sequences flanking the − 35 and − 10 box of the P ylb promoter were divided into six segments, and six random-scanning mutant promoter libraries fused to an enhanced green fluorescent protein EGFP were made and analyzed by flow cytometry. Our results showed that the four nucleotides flanking the − 35 box could mostly influence the promoter activity, and this influence was related to the GC content. The promoters mutated in these regions were successfully used for expressing the gene ophc2 encoding organophosphorus hydrolase (OPHC2) and the gene katA encoding catalase (KatA). Conclusion Our work identified and characterized the sequence signatures of the P ylb promoter that could tune the promoter strength, providing further information for the potential application of this promoter. Meanwhile, the sequence signatures have the potential to be used for tuning gene expression in enzyme production, metabolic engineering, and synthetic biology.
Type of Medium:
Online Resource
ISSN:
0141-5492
,
1573-6776
DOI:
10.1007/s10529-019-02749-4
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2020
detail.hit.zdb_id:
2012203-2
SSG:
12
