In:
Plant Cell, Tissue and Organ Culture (PCTOC), Springer Science and Business Media LLC, Vol. 152, No. 1 ( 2023-01), p. 201-206
Abstract:
Sphagnum mosses are important carbon sequesters and emerging model organisms. However, induction and long-term cultivation of thalloid protonema in several species was not achievable so far. Here, we provide protocols for a set of new tools relevant for Sphagnum molecular biology: a new way for Sphagnum protoplast isolation and regeneration, and a first protocol for transient protoplast transformation. Together, these protocols will support the emerging Sphagnum research community in basic and applied science.
Type of Medium:
Online Resource
ISSN:
0167-6857
,
1573-5044
DOI:
10.1007/s11240-022-02384-4
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2023
detail.hit.zdb_id:
1478391-5
SSG:
12
