In:
Lipids, Wiley, Vol. 38, No. 11 ( 2003-11), p. 1133-1142
Abstract:
We explored whether CLA isomers and other C 18 FA affect (i) lipid content and FA concentrations in total adipocyte lipids, (ii) FA synthesis from glucose in TAG and phospholipids of primary brown (BAT) and white adipocytes (WAT), and (iii) mRNA expression of uncoupling protein 1 (UCP1) in primary brown adipocytes of Djungarian hamsters ( Phodopus sungorus ). c 9, t 11‐CLA, oleic, linoleic, and α‐linolenic acid increased whereas t 10, c 12‐CLA decreased lipid accumulation in both adipocyte types. t 10, c 12‐CLA treatment affected FA composition mainly in BAT cells. CLA incorporation into lipids, in particular c 9, t 11‐CLA, was higher in BAT. In both cell types, t 10, c 12‐CLA treatment reduced the incorporation of glucose 13 C carbon into FA of TAG and phospholipids, whereas c 9, t 11‐CLA, linoleic, and α‐linolenic acid either did not influence or dose‐dependently increased glucose carbon incorporation into FA. UCP1 mRNA expression was inhibited by t 10, c 12‐CLA but increased by c 9, t 11‐CLA, linoleic, and α‐linolenic acid. It is concluded that c 9, t 11‐CLA and t 10, c 12‐CLA have distinctly different effects on lipid metabolism in primary adipocytes. The effects of c 9, t 11‐CLA are similar to those of other unsaturated C 18 FA. The opposite effects of c 9, t 11‐CLA and t 10, c 12‐CLA are evident in both WAT and BAT cultures; however, brown adipocytes seem to be more susceptible to CLA treatment.
Type of Medium:
Online Resource
ISSN:
0024-4201
,
1558-9307
DOI:
10.1007/s11745-003-1171-0
Language:
English
Publisher:
Wiley
Publication Date:
2003
detail.hit.zdb_id:
2030265-4
SSG:
12